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Your should check, that the peaks do not move at the different experiments. Try opening some random spectra, and overlay them in SPARKY.<br>
Read the section in [[Tutorial_for_Relaxation_dispersion_analysis_cpmg_fixed_time_recorded_on_varian_as_fid_interleaved#Check_for_peak_movement | Check for peak movement]].
== Measuring peak heights ==
We will use the program [[NMRPipe_seriesTab | NMRPipe seriesTab]] to measure the intensities.
'''seriesTab''' needs a input file, where the ppm values from a [[SPARKY_list | SPARKY list]] has been converted to spectral points.<br>
The spectral points value depends on the spectral processing parameters.
=== Generate spectral point file ===
Create a file with spectral point information with script
[[Tutorial_for_Relaxation_dispersion_analysis_cpmg_fixed_time_recorded_on_varian_as_fid_interleaved_scripts#stPeakList.pl | stPeakList.pl]] .
<source lang="bash">
stPeakList.pl 0.fid/test.ft2 ../peak_lists/peaks_corr_final.list > peaks_list.tab
cat peaks_list.tab
</source>
=== Make file with paths to .ft2 files ===
Then we make a file list of filepaths to .ft2 files.
<source lang="bash">
ls -v -d -1 */*.ft2 > ft2_files.ls
cat ft2_files.ls
</source>
=== Measure the height or sum in a spectral point box ===
<source lang="bash">
seriesTab -in peaks_list.tab -out peaks_list_max_standard.ser -list ft2_files.ls -max
seriesTab -in peaks_list.tab -out peaks_list_max_dx1_dy1.ser -list ft2_files.ls -max -dx 1 -dy 1
</source>
OR make the sum in a box:
<source lang="bash">
seriesTab -in peaks_list.tab -out peaks_list_sum_dx1_dy1.ser -list ft2_files.ls -sum -dx 1 -dy 1
</source>
