Difference between revisions of "DASHA"

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m (Bugman moved page Dasha to DASHA: Match the capitalisation used by the original authors.)
(Changed the sectioning and listed the relax user functions for DASHA.)
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The software DASHA is designed for the model-free analysis for NMR relaxation data.  DASHA can be used as an optimisation engine to replace the minimisation algorithms implemented within relax.
 
The software DASHA is designed for the model-free analysis for NMR relaxation data.  DASHA can be used as an optimisation engine to replace the minimisation algorithms implemented within relax.
  
== Website ==
+
== Details ==
 +
 
 +
=== Website ===
  
 
The original DASHA website is http://www.nmr.ru/dasha.html (and [https://web.archive.org/web/20150223190004/http://www.nmr.ru/dasha.html archived on the Internet Archive]).
 
The original DASHA website is http://www.nmr.ru/dasha.html (and [https://web.archive.org/web/20150223190004/http://www.nmr.ru/dasha.html archived on the Internet Archive]).
  
== Overview ==
+
=== Overview ===
  
 
From the DASHA homepage:
 
From the DASHA homepage:
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{{quote|text=DASHA is an interactive program designed to investigate dynamics of biomolecules, for which data of <sup>15</sup>N or <sup>13</sup>C heteronuclear relaxation are available from NMR measurements. A number of sets of longitudinal and transverse relaxation rates of <sup>15</sup>N or <sup>13</sup>C nuclei and <sup>1</sup>H-<sup>15</sup>N, <sup>13</sup>C NOE's obtained at various NMR spectrometer frequencies might be used as the input. The measured longitudinal, transverse relaxation rates and NOE values are interpreted using the model-free approach of Lipari and Szabo (1982, J. Am. Chem. Soc., 104, 4546-4559). In addition to the overall rotational correlation time of the molecule, the internal dynamics of backbone N-H or C-H vectors of two types of internal motions - fast, on a time scale of &lt;20 ps, and intermediate, close to 1 ns could be evaluated by constructing correspondent spectral density functions. Contribution of the conformational exchange to transverse relaxation rates of individual nitrogens or carbons could be elucidated using a set of different rates of the CPMG spin-lock pulse train in measuring {{:T2}} relaxation times (Orekhov et al., 1994, Eur. J. Biochem., 219, 887-896). Separate module DIFFC, included into the DASHA software, performs hydrodynamic calculations for proteins with known spatial structure. All input and output data could be easily presented in PostScript format.}}
 
{{quote|text=DASHA is an interactive program designed to investigate dynamics of biomolecules, for which data of <sup>15</sup>N or <sup>13</sup>C heteronuclear relaxation are available from NMR measurements. A number of sets of longitudinal and transverse relaxation rates of <sup>15</sup>N or <sup>13</sup>C nuclei and <sup>1</sup>H-<sup>15</sup>N, <sup>13</sup>C NOE's obtained at various NMR spectrometer frequencies might be used as the input. The measured longitudinal, transverse relaxation rates and NOE values are interpreted using the model-free approach of Lipari and Szabo (1982, J. Am. Chem. Soc., 104, 4546-4559). In addition to the overall rotational correlation time of the molecule, the internal dynamics of backbone N-H or C-H vectors of two types of internal motions - fast, on a time scale of &lt;20 ps, and intermediate, close to 1 ns could be evaluated by constructing correspondent spectral density functions. Contribution of the conformational exchange to transverse relaxation rates of individual nitrogens or carbons could be elucidated using a set of different rates of the CPMG spin-lock pulse train in measuring {{:T2}} relaxation times (Orekhov et al., 1994, Eur. J. Biochem., 219, 887-896). Separate module DIFFC, included into the DASHA software, performs hydrodynamic calculations for proteins with known spatial structure. All input and output data could be easily presented in PostScript format.}}
  
== Authors ==
+
=== Authors ===
  
 
* Vladislav Yu. Orekhov
 
* Vladislav Yu. Orekhov
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* Alexander S. Arseniev.
 
* Alexander S. Arseniev.
  
== Version ==
+
=== Version ===
  
 
The last release was 3.48c in May 2000.
 
The last release was 3.48c in May 2000.
  
== Reference ==
+
=== Reference ===
  
 
* {{#lst:Citations|Orekhov95}}  
 
* {{#lst:Citations|Orekhov95}}  
 +
 +
== Usage in relax ==
 +
 +
To use DASHA within relax, the following user function are provided:
 +
 +
* [http://www.nmr-relax.com/manual/dasha_create.html dasha.create]
 +
* [http://www.nmr-relax.com/manual/dasha_execute.html dasha.execute]
 +
* [http://www.nmr-relax.com/manual/dasha_extract.html dasha.extract]
 +
 +
These allow DASHA to be used as a model-free optimisation within relax, replacing the built-in algorithms.
  
 
== See also ==
 
== See also ==
  
 
[[Category:Model-free analysis]]
 
[[Category:Model-free analysis]]

Revision as of 15:16, 15 December 2015

The software DASHA is designed for the model-free analysis for NMR relaxation data. DASHA can be used as an optimisation engine to replace the minimisation algorithms implemented within relax.

Details

Website

The original DASHA website is http://www.nmr.ru/dasha.html (and archived on the Internet Archive).

Overview

From the DASHA homepage:

DASHA is an interactive program designed to investigate dynamics of biomolecules, for which data of 15N or 13C heteronuclear relaxation are available from NMR measurements. A number of sets of longitudinal and transverse relaxation rates of 15N or 13C nuclei and 1H-15N, 13C NOE's obtained at various NMR spectrometer frequencies might be used as the input. The measured longitudinal, transverse relaxation rates and NOE values are interpreted using the model-free approach of Lipari and Szabo (1982, J. Am. Chem. Soc., 104, 4546-4559). In addition to the overall rotational correlation time of the molecule, the internal dynamics of backbone N-H or C-H vectors of two types of internal motions - fast, on a time scale of <20 ps, and intermediate, close to 1 ns could be evaluated by constructing correspondent spectral density functions. Contribution of the conformational exchange to transverse relaxation rates of individual nitrogens or carbons could be elucidated using a set of different rates of the CPMG spin-lock pulse train in measuring T2 relaxation times (Orekhov et al., 1994, Eur. J. Biochem., 219, 887-896). Separate module DIFFC, included into the DASHA software, performs hydrodynamic calculations for proteins with known spatial structure. All input and output data could be easily presented in PostScript format.

Authors

  • Vladislav Yu. Orekhov
  • Dmitry M. Korzhnev
  • Dmitry E. Nolde
  • Alexander P. Golovanov
  • Alexander S. Arseniev.

Version

The last release was 3.48c in May 2000.

Reference

  • Orekhov, V. Y., Nolde, D. E., Golovanov, A. P., Korzhnev, D. M. and Arseniev, A. S. (1995). Processing of heteronuclear NMR relaxation data with the new software DASHA Appl. Magn. Reson., 9(4), 581-588. (DOI: 10.1007/bf03162365)

Usage in relax

To use DASHA within relax, the following user function are provided:

These allow DASHA to be used as a model-free optimisation within relax, replacing the built-in algorithms.

See also